According to Heroic Hollywood, Marisa Tomei filmed a cameo as Aunt May for Captain America: Civil War. This is in addition to the expected debut of Tom Holland as Peter Parker/Spider-Man.
The film also stars Chris Evans, Robert Downey Jr, Scarlett Johansson, Anthony Mackie, Paul Bettany, Jeremy Renner, Don Cheadle, Elizabeth Olsen, Sebastian Stan, Chadwick Boseman, Emily VanCamp, Daniel Bruhl, Frank Grillo, William Hurt, Paul Rudd and Martin Freeman. Look for Captain America: Civil War in theaters May 6, 2016.